Leucocytes, urea and glucose levels in Albino Wistar rats exposed to doses of isolated Achatina achatina snail lectin

There are five (5) types of mature White blood cells (WBC) or Leucocytes found in the peripheral viz, Neutrophils (NEU), Eosinophils (EOS) and Basophils (BAS) (granulocytes); Monocytes (MON) Lymphocytes (LYM) (agranulocytes). Urea is an organic chemical compound, essentially waste produced by body after metabolizing protein. levels can be used to detect diseases disorders that affect kidneys. A common disease related irregular management glucose diabetes. Lectins proteins recognize specifically bind reversibly carbohydrate-containing molecules foreign elicit diverse physiological responses various organisms. total 120 samples Nigeria Achatina achatina snail specie were collected, authenticated at Zoology Department University Nigeria, Nsukka 80mls pooled crude Lectin extract was obtained. Purifications performed on 20mls three steps Ammonium sulphate precipitation Dialysis (Partial purifications), Con Sepharose 4B affinity Chromatography column (Complete purification). The purified for all tests conducted this research. crude, partially complete/affinity extracts subjected Haemagglutination tests. further assessed determine its effects Leucocytes, Glucose as follows: Thirty-five (35) male Albino Wistar Rats weighing 101-180g aged 2-3 months obtained from Animal house Nsukka, animals Grouped into 5 (A-E) allowed 2 weeks acclimatization. Graded doses 0.04ml, 0.05ml 0.06ml Affinity injected intra-peritoneally each Groups A-D (test groups) according their weights intervals days 1 week. Group E served control. Two (2) mls collected before 24 hours last day Doses injections following tests: WBC-Total Differential counts (using Sysmex Corporation, 1999 automated equipment), estimations (performed means Urease-Berthelot GOD-PAP Randox Monza analyser methods respectively). results research showed On purification, 15mls pure sample containing only high molecular weight haemagglutination standardization preferential agglutination with Blood group type. Bar Charts statistics show there Post mean increase Total WBC, NEU, LYM decrease MON, EOS, BAS, levels. However, differences Pre values these parameters One way analysis variance (ANOVA) test if statistically significant. ANOVA LYM, insignificant. EOS This has therefore succeeded Assessment Activities A.